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Mouse anti-Rat IgG2a (Chain gamma 2a) Antibody

FACS, IF, EIA Monoclonal 2A 8F4 IgG1 unconjugated
Catalog No. ABIN371227

Quick Overview for Mouse anti-Rat IgG2a (Chain gamma 2a) Antibody (ABIN371227)

Target

IgG2a

Clonality

  • 70
  • 30
Monoclonal

Application

  • 61
  • 43
  • 42
  • 37
  • 24
  • 19
  • 17
  • 11
  • 10
  • 9
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Flow Cytometry (FACS), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone

2A 8F4

Isotype

IgG1
  • Binding Specificity

    • 41
    • 8
    • 7
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Chain gamma 2a

    Reactivity

    • 98
    • 20
    • 1
    Rat

    Host

    • 56
    • 28
    • 15
    • 15
    • 5
    Mouse

    Purification

    Purified
  • Application Notes

    Flow Cytometry. Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells. ELISA. Second step reagent for rat IgG2a Monoclonal Antibodies.

    Other applications not tested.
    Otimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

    Preservative

    Without preservative

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
  • Target

    IgG2a

    Target Type

    Antibody

    Background

    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). The IgG2a Ig subclass plays a critical role in the pathogenesis of humoral auto-immunity and protection against pathogens.Synonyms: Rat Immunoglobulin G2a
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